Features: Standard back plate for use with hand sanitizer, hand lotion, or foam dispensers. Incubation to 70 o C enables high temperature assays such as enzyme kinetics, protein stability/aggregation. Specifications subject to change without notice. Detect dim samples with unsurpassed sensitivity; Avoid saturation issues with nine orders of dynamic range. Part Number. The CellTiter-Glo assay was used to measure ATP. flat-bottom: 50-60 µl. Fluorescence Microplate Reader Comparison Chart. The MultiWash+™ Microplate Washer is an automated, compact, quiet, efficient washer with 20 different wash protocol options. It also integrates with the. • Gently wash cells once with 200 μl (for 96 well plate) or 400 μl (for a 24 well plate) warm (37°C) 1X DPBS (containing Mg and CaCl 2). Step 4: Add detection antibody – Enzyme-conjugated detection antibody binds to a second site on the. With various on-board features and capabilities, the ThermoMax is ideal for use in modern laboratories involved in many applications. 4 (Molecular Devices cat. com Molecular Devices (Germany) GmbH ismarcring ierach an der iss erman ©2019 Molecular Devices, LLC. 5 pM 1 0200-7015POS Glow for 96- and 384-well plates 96–well: 1. SoftMax Pro GxP Software version 5. x. SpectraTest FL1 Fluorescence Validation Plate User Guide Molecular DevicesData are shown as the mean ± s. Category 2 and 3 microplate readers use gradient heating as well. xThe SpectraMax® M3 Microplate Reader is the standard for UV/visible multi-mode reader absorbance, providing ultrafast, full spectral range detection for cuvettes and 6-, 12-, 24-, 48-, 96-, and 384-well microplates. Absorbance Plate Reader Comparison Chart. Fluorescence, Absorbance Fastest Read Time - 96-well plate: 20 sec; 384-well plate: 30 sec Temperature Control: Ambient +5C up to 42C Shaking: Linear and Orbital. Molecular Devices FMax Thermo Fluoroskan Ascent Fluorescence Microplate Reader W WARRANTY . Therefore you need to ask a physicist. OD measurements of bacterial and colloidal bead cultures were performed in a Spectrostar Omega microplate reader (BMG, Germany) with a Costar Flat Bottom 96-well plate with lid and 200 µl per. SpectraMax and VersaMax Plate Readers Operator’s Manual — 0112-0126 Rev A SpectraMax Plus 384. Robust shaking and consistent temperature control optimize bacteria and yeast cell growth. Infinite possibilities. This 96-well assay has been fully validated on the SpectraMax iD5 reader and other Molecular Devices microplate readers with FP detection to ensure reliable results. 0200-6263. This instrument is ideal for measuring dual luciferase assays in both 96- and 384-well plates, and SoftMax Pro® software simplifies data collection and analysis with preconfigured protocols. 46Click the Read button in the SoftMax Pro3 reader combines Molecular Devices SpectraMax® M5e Multi-Mode Microplate Reader performance with an integrated 8- or 16-channel pipettor into one compact. 96-, 384-, and 1536-well microplates. • 96-well yellow plate 0200-6182 Pipettor head kit, 16-channel (384) for FlexStation 3 Reader • 16-channel pipettorElisa Plate Readers and Microplate Readers at Biocompare. 0025 OD from 0. UV Plate 96 Well, Flat Bottom, Cs 50. Molecular Devices has provided scientists with . Select up to 3 products. Robust, high-value microplate readers that can run samples based on pre-defined protocols and standard filter modes cover the entire visible range for a variety of assays. The clear UltraVision FC 96-well microplate (FC_UltraVision (clear, 96)) showed the best performance of the tested microplates, with absorbance of 0. Make sure toPlate reader instructions Computer. EMax. A standard curve consisting of MCF7 cells seeded from 20 to 20,000 per well in a 96-well plate was linear across the entire range with r 2 = 0. Z. Well-to-WellPrecision RFULinearity RelativeFluorescenceUnit(RFU)ScaleRatio(GeminiEM,XPS,SpectraMaxM2-M5e, FlexStation3only). Detection modalities include absorbance (UV-Vis Abs), fluorescence intensity (FI), luminescence (Lumi), and all have. Transfer the assay plate directly to a bottom-read fluorescence microplate reader for kinetic read-mode or incubate for 10-30 min at 37oC and then read for endpoint read-mode. The SpectraMax i3x Multi-Mode Microplate Reader has been validated for use with the ValitaTiter and ValitaTiter Plus assays. 313 reference gain check fail. Published Dec 16,. Other technologies such as imaging. Plate formats 6, 12, 24, 48, 96, 384 wells Light source Xenon flash lamp (1 joule/flash) Detector Photomultiplier (R-3896). Browse for different fluorescent readers below and compare across different instrument suppliers. The reduced cost of the device represents the major technical contribution of this manuscript, as costs were reduced 7 fold from previous reports. Robust, high-value microplate readers that can run samples based on pre-defined protocols and standard filter modes cover the entire visible range for a. 200, the Bio-Tek µQuant™, the Bio-Rad UltraMark™, the Molecular Devices SPECTRAmax® 190 and 250 plate readers. Instruments with a 96- or 384-well format will accommodate the most commonly used plates. Chirag Patel, a graduate student at Johns Hopkins, reviewed Molecular Devices' SpectraMax M5 Multi-Mode Microplate Reader and found it an excellent resource for his lab's research on mTOR signaling in CD8 T cells. Plus 384. Erik Koppes, Richard Haas, Jerry Vockley, Lina Ghaloul-Gonzalez approximately 5,000 cells were seeded in 4 replicates in 96-well plates. Robust, high-value microplate readers that can run samples based on pre-defined protocols and standard filter modes cover the entire visible range for a variety of assays. Place the microplate in the drawer. Microplate Reader. Published Dec 16, 2020 | Updated Dec 16, 2020. The BioTek Synergy H1 multimode microplate reader is modular and upgradable to include variable-bandwidth monochromator optics for fluorescence excitation and emission wavelength selection, plus luminescence and UV-Vis absorbance. Suggested plates are black walled. Polypropylene deep well plates, round bottom. Two high-energy xenon flash lamps: Reading capabilities. Step 3: Wash microplate – Unbound material is washed away, leaving only the antigen of interest. 993 (Figure 3), validating the assay’s usefulness over a wide range of viable cell numbers. Top read: purple adapter (p/n: 0310-4336) Bottom read: black adapter (p/n: 0310-4089) Flex mode always uses black adapter because it's always bottom read. The plate reader optics are tunable for absorbance, fluorescence intensity, fluorescence polarization, and time resolved fluorescence assays (TRF). 2% in. Fireflysci UV/VIS Fluorescence Validation Plate for Well Plate Reader (290-562nm) FLVP-01. None for any other plate (6, 12, 24, 48 well plates) Flex3: Both Top read and Bottom read. Monthly Plate Verification Check (Absorbance Validation Plate) Note: Monthly Plate Verification Check is maintained by Quality Control. With the novel filter system, assays such as hydrogen peroxide detection assays and IRantibody-binding assays can be run confidently with Molecular Devices. 0 nm increments Dynamic range > 7 decades Cross-talk < 0. 96-well Molecular Devices P/N 42-000-0117 HE™ High Efficiency microplates, 384-well Molecular Devices P/N 0200-5201. For a higher degree of automation, the Automation Vendor Partners Program has streamlined the integration of our microplate reader systems with all leading partner robots. High tolerance for contaminants. $14,995. Including injection time, it takes approximately 68 secs. x. Microplate readers compatible with 96-well plates Single- to multi-mode readers combine an absorbance plate reader , a luminescence plate reader , and a fluorescence plate. Anticancer drug assay was also conducted using conventional 96-well plates as controls. one 96-well sample plate can be controlled from 15–40°C,. AlphaScreen and AlphaLisa detection using a 680 nm excitation laser diode and a 570/100 nm emission filter. Single or dual wavelength read, kinetic readings, automix, endpoint, and blank pattern, curve fits included with PC Software. Making it possible to perform kinetic analysis of solid. Enable more assay conditions Define individual reagents to be delivered to each well reducing reagent consumption and allowing for more assay conditions to be explored on a single microplate. The Absorbance 96 Plate Reader is designed to run everyday assays with ease and speed. Molecular Devices: EMax Plus: SpectraMax 190: SpectraMax 340PC384: SpectraMax Plus384: VERSAmax: Vmax: Tecan: Sunrise:. Please refer to the Obsolete Microplate Reader Models for more information. The drawing shows a standard microplate of 15 mm height covered with a lid. Products : SpectraTest Validation Plates ABS2, SpectraTest Validation Plates ABS1, SpectraTest Validation Plates FL1, SpectraTest Validation Plates LM1. This study investigated the efficacy of fermented seaweed (Eucheuma cottonii) on the remediation of fluoride-contaminated soil. This is equivalent to having 511 built-in filters, The instruments offer 96- and 384-well microplate-to-microplate fluid transfer (8 or 16 wells at a time), and can read microplates having 6, 12, 24, 48, 96, and 384-well formats. Turn the instrument around so that the back of the instrument is. The VersaMax Microplate Reader is supplied with SoftMax® Pro Data Acquistion & Analysis Software, Molecular Devices' industry leading all-in-one data acquisition and analysis. 150 µL of deionized water was pipetted into each well of a clean 96-well plate. 96-well plate: 0 to 4 Abs, ±2% at 450 nm, 384-well plate: 0 to 3 Abs ±2% at 450 nm: Measurement Type: Endpoint, Kinetic, Spectra, Multipoint, Kinetic Spectra: No. Top and bottom reads are. No. 3% in white 96- and 384. Products : SpectraMax M2/M2e reader, SpectraMax M5/M5e reader, Gemini XPS reader, FlexStation II (Obsolete), Gemini EM reader, FlexStation 3 reader. x. Up to 96 well plates, there is often a good signal that can easily be detected, and 96 wells are measured comparatively quickly. VersaMax Microplate Spectrophotometer Operator's Manual Molecular Devices Corporation 1311 Orleans Drive Sunnyvale, California 94089 Part # 0112-0126 Rev A. Narrow bandwidths deliver increased measurement accuracy and linearity over the widest range of assays, including. Partner with our team of scientists and engineers who can customize your instruments, as well as automate entire workflows to meet the specific needs of your assay, method, or protocol. These microplates have a corner notch for use in standard plate readers. Instrument settings can beCorning 96-well black microplates with clear bottom (Corning cat. com. 5in (46. Instrument settings can. Thermo Scientific Pierce Streptavidin Coated Plates are pre-blocked and ready-to-use polystyrene plates for binding biotinylated antibodies or probes for ELISA and other target-specific assays. Upon completion of a plate read, data are delivered to your computer in an Excel-compatible format. 100 fmol 384-well (75 µL) 0. 000 – 4. These robust readers have been placed in labs from Antarctica to. Recertification service. Page 20: Plate Adapter SpectraMax M3, M4, M5, and M5e Microplate Reader User Guide Plate Adapter Before you read standard 96-well or 384-well plates from the top, you must insert the plate adapter in. minutes and begin reading microplates with seven mouse clicks. At 5 seconds to read a 96-well endpoint assay or a 5 second minimum interval between kinetic reads the VMax Microplate Reader has both speed and performance. The SpectraMax® M3 Multi-Mode Microplate Reader provides three modes of detection in one platform. Another consideration, says Amouretti, is plate format. x. #E010) Pyroplate 96-well microplate (ACC cat. Standard features include a cuvette port, spectral scanning in 1 nm increments, and up to six wavelengths per read. These are the default kind of cuvette & 96 well plate stocked by the lidstrom lab as of 11/2013. 5. : 2 Oct 12 Page 1 of 20 Setup Guide on the Molecular Devices SpectraMax® Paradigm® Microplate Detection Platform Have a question? Contact our Technical Support Team NA: 800-955-6288 or INTL: 760-603-7200 ext. Standards, controls and blanks were pipetted into the wells and incubated followed by washing with the Molecular Devices MultiWash™+ Microplate Washer. Enable more assay conditions Define individual reagents to be delivered to each well reducing reagent consumption and allowing for more assay conditions to be explored on a single microplate. An additional reference channel for optimizing the light intensity; Auto switch-off of the light source when not in use to extend lifetime; An automated self-check of major components at instrument start-upThe EMax® Plus Microplate Reader delivers a robust solution in an entry level platform. Packed 25 plates per…. 100 fmol luciferase per well. The SpectraMax ABS readers can be integrated with Molecular Devices StakMax® Microplate Stacker within . The plate was incubated for 10 minutes at room temperature and read at 595 nm on the SpectraMax ABS Plus reader. However, for greater flexibility, some instruments can accommodate 6- to 1536-well plate formats. We would like to show you a description here but the site won’t allow us. 5039228D 3 Contents SafetyInformation 5 Warnings,Cautions,Notes,andTips 5 Chapter1:Introduction 11 ComputerIntegration 12 Applications 12 Optics 13 PlateControls 13The microplate reader has been around for more than three decades and continues to serve as a must-have workhorse of clinical, academic research and industrial laboratories alike. Two different agonists, acetylcholine and carbachol, were tested. The patented quench technology is offered to drug discovery and life science researchers exclusively by Molecular Devices, through the purchase of FLIPR Assay Kits. SpectraMax Paradigm Microplate Reader General specifications User-upgradeable Multi-Mode Detection Platform Technical specifications Optimized Guaranteed* Fluorescence Intensity (FI) (Fluorescein) 96-well (200 µL) 0. the Molecular Devices SPECTRAmax® 190 and 250. Published Dec 16, 2020 | Updated Nov 07, 2022. * Note: Programming the washer with a final aspirate height of 4. This is a Molecular Devices Thermomax 96 well Absorbance Microplate Readers. (96- or 384-well) simultaneously and with high temporal resolution. Reaction formats The MagMAX™-96 DNA Multi-Sample Kit can accommodate a broad range of throughputs. The SpectraMax ABS/ABS Plus Microplate Readers provide fast absorbance detection without the use of filters with monochromator-based wavelength selection for visible and. Click Create. Categories: Plate Readers. Supersedes 1. Whether running fluorescence imaging or brightfield assays, the automated imager features a comprehensive portfolio of preconfigured protocols for cell-based assays to shorten the learning curve, so. where I 0 is the intensity of the incident light, and I is intensity of that light after it passed through the sample. Standard features include a cuvette port, spectral scanning in 1 nm increments, and up to six wavelengths per read. Read up to 2 wavelengths in endpoint absorbance for 96-well plates. After incubation of the cells with compounds, add 100 µL (for 96-well plates) or 25 µL (for 384-well plates) of Dye Solution per test well. The SpectraMax M5e Micoplate Reader is the standard for UV/Visible multi-mode reader absorbance, providing ultrafast, full spectral range detection for cuvettes, 96-well and 384-well microplates. Explore the best practices for running absorbance-based assays how Molecular Devices absorbance readers can be utilized to optimize said absorbance-based assays. Molecular Devices delivers a robust screening platform to address real-time kinetic cell-based assays with the FLIPR Tetra® System. ©2017 Molecular Devices, LLC 3/17 0120-1441I Printed in USA Figure 3. Cells were transfected as previously described and plated at 30,000 cells per well in a 96-well plate and grown overnight. By measuring optical pathlength, it eliminates the last major distinction between microplate readers and spectrophotometers. Compact design Minimum volume and maximum throughput with 96- or 384-well microplate compatibility Powerful data analysis with SoftMax® Pro Software Optimal performance with validation tools Small but Mighty EarlyTox Live/Dead Assay Kit (Molecular Devices cat. Using the patented PathCheck® Pathlength Measurement Technology, the SpectraMax M4 Microplate Reader transforms each well in a microplate to a fixed. The perfect microplate reader for everyday biochemical assays, cell-based assays, molecular biology and immunoassays. Microplates, also known as microtiter plates, are rectangular multi-well plates used in a variety of assays from ELISA to PCR. 984. A = Log 10 (I 0 /I). To maximize sensitivity of the assay, it is necessary to use optimal excitation and emission wavelengths. absorbance microplate reader GEA. Download a PDF version of the SpectraTest validation package User Guide and the ABS2 SpectraTest validation protocols. Detection modalities include absorbance (UV-Vis Abs) and fluorescence intensity (FI). FAST AND ACCURATE METERTECH Microplate Reader $3,650. is required for optimum performa nce with standard 96-well and 384-well format microplates for all top-read applications. Enables data acquisition in endpoint, kinetic, and spectral scan modes at wavelengths across the UV-visible wavelength range, with the only temperature-independent method of pathlength correction. #23225). For a 96-well plate it takes a total of 27 seconds to switch between wells and then you have to add the actual injection time based on the injection rate entered by the user. x. Page 1 of 8 . 1. The purpose of this procedure is to set instructions in the proper use and handling of the Molecular Devices M5 Plate Reader. 2. Clear plates will give more cross talk between wells. 057, depending on lot number. Plate Shaking. Learn more. Resipher 96x Starter Kit for use with 96-well plates Includes: (1) Resipher Data Hub, (1) Resipher 96x Reader, (3) sterile sensing lids, all cables Turns a 96-well plate into a precise dynamic oxygen consumption reader, measures 96 wells Utilizes Lucid Lab Cloud software for connection to any…. 20 µL (96-well plates) or 10 µL (384. \r Our multi-mode plate readers provide great flexibility and include absorbance, fluorescence and luminescence with configurable options for fluorescence polarization (FP), time. For a higher degree of automation, the Automation Vendor Partners Program has streamlined the integration of our microplate reader systems with all leading partner robots. Compare. #R8195. maximum-illumination-channels. Temperature control: 4°C above ambient to 45°C. 0 OD. 5 ng per well. Both COC microplates, the μCLEAR UV STAR 96- and 384-well plates, had background absorbance of 1. Running both standard spectrophotometer and microplate. For the name of the nearest authorized Molecular Devices Corporation service facility, please contact Molecular Devices at one of the following telephone numbers: (408) 747-1700 (800) 635-5577 (U. 1 – 2. SpectraMax ID5 Plate Reader (Molecular Devices) Description Internal price (Cornell and Cornell affiliates) External Price; SpectraMax ID5 Plate Reader Use: $20/hr: $32/hr. #ABS PLUS) Methods. Temperature variation ± 0. What plates do you recommend for SpectraMax Plate Readers? Luminescence: Solid white plates for top reads and white plates with clear bottoms for bottom reads. The variations include adjustable speed and volume, adjustable aspiration speed and time, adjustable soak times, and three modes of shaking. Compare this item. Source plate capacity; 1 x plates. Answer. 100 μL of working reagent was added to each well after the 10-minute preincubation step. 306 plate air cal. A preconfigured protocol with optimized reader settings enabled reading of a 384-well plate in just two minutes. Validation made easy -1) Select the correct plate and the wells that you want to measure. Microplate Spectrophotometer Operator's Manual Molecular Devices Corporation 1311 Orleans Drive Sunnyvale, California 94089 Part # 0112-0126 Rev A. The Molecular Devices Gemini EM is built on the Gemini platform delivering high quality top and bottom reading for both solution and cell based assays. 5°C at 37°C. The SpectraMax® Mini Multi-Mode Microplate Reader offers a budget-friendly solution with three modes of detection for UV-Vis absorbance, fluorescence, and luminescence. Please click on the links below to view the Microplate Reader Comparison Tables. Fluorescence Microplate Reader Comparison Chart. The reader can measure flat and round 96-well microplates and ensure accuracy by automatic lamp calibration before each reading. perfect for kinetic readings involving growth curves at 5 seconds per plate. 10 µL of BSA standard or cell lysate was pipetted into wells of a 96-well microplate (n = 3). 1. The SpectraMax® iD3 Multi-Mode Microplate Reader is the cornerstone of a complete laboratory solution designed to expand the boundaries of research capabilities. U-bottom: 30-40 µl. This reader's dual monochromators for. An entire 96-well plate can be processed in less than six seconds. D. Transfer the assay plate directly to a bottom-read fluorescence microplate reader for kinetic read-mode or incubate for 10-30 min at 37oC and then read for endpoint read-mode. The SpectraMax® i3x Multi-Mode Microplate Reader measures absorbance, fluorescence, and luminescence with available upgrades including western blot, cell imaging, and fast kinetics with injectors plus additional detection modes. 90349). 384-WELL SBS STERILE MICROPLATE. Figure 3. For FlexStation I/II/3 plate readers, what are the dead-volumes for the compound plate? The FlexStation plate readers assume a 1 mm pipette height from the bottom of the well when aspirating from a compound source plate. 00. as well as all other Molecular Devices' microplate readers. SpectraMax® iD5 Multi-Mode Microplate Reader - Five-Mode Hybrid Microplate Reader with Automatic NFC Filter Identification and Western Blot Capability, Molecular Devices. The SpectraMax ABS readers can be integrated with Molecular Devices StakMax® Microplate Stacker within . Because many reagents can be fluorescently labeled, spectrofluorometers are used to detect many biological and chemical processes. 2. Kinetic assays were read every 20-28 seconds for one hour,The SpectraMax® M Series Multi-Mode Microplate Readers measure UV and visible absorbance, fluorescence, luminescence, fluorescence polarization, TRF and HTRF. 5L) MultiWash+. Expand Microplates and select the newly created plate. 000 OD ± 1. Products : SpectraMax L reader How long does it take to dispense into a 96 well plate with SpectraMax L reader injection? For a 96-well plate it takes a total of 27. 5 mm left a residual volume of 100 µL in each well of a 96-well plate or 25 µL for a 384-well plate. 315 can't find zero order. We would like to show you a description here but the site won’t allow us. UV absorbance detection. and the A1 corner of the plate adapter (Figure 1-2). Molecular Devices L-Max II Luminescence Microplate Reader. BioTek LogPhase 600 microbiology reader measures microbial growth curves in up to four standard 96-well microplates at a time. VersaMax Microplate Spectrophotometer Operator's Manual Molecular Devices Corporation 1311 Orleans Drive Sunnyvale, California 94089 Part # 0112-0126 Rev A. 5 OD at 492 nm Computer interface USB 2. Quantitative, whole well cell confluence for every well. Each wellThe Molecular Devices ThermoMax is an efficient, temperature-regulated, 96 well microplate reader. x. External Dimensions without lid (mm). Reads various kinds of 96-wells plates, and is equipped with shaking function. 0200-6262. Shaking: Linear, orbital, and double orbital. The soil was amended with either 1. A 1:10 dilution series of ATP was set up in solid white 96- and 384-well plates at concentrations from 10 uM down to 1 nM, along with blank wells containing no cells. Prior to the reading of each plate, the microplate reader was “blanked” with a plate containing 0. Before dispensing reagents to the microplate, the VersaMax Reader was powered on and. Dual injectors for kinetic assays. The SpectraMax® M2 and M2e Systems from Molecular Devices are multi- detection microplate readers with dual- monochromators, dual-mode cuvette ports, and top- and. To help you get the best results from your Molecular Devices® plate readers it is essential that you choose the right plate. With various on-board features and capabilities, the ThermoMax. Washers configured for both 96- and 384-well plates. g. In addition, users can define individual reagents and concentrations to be delivered to each well. Molecular Devices' microplate readers. More Product Information. Here, we demonstrate that with Molecular Devices SpectraMax® microplate readers and the Quant-iT PicoGreen assay, users can reliably measure concentrations as low as 50 pg/mL of double-stranded DNA. The screen on the left shows the wells selected for Method MI AF488-10 and Method MI AF488-5 for image acquisition from rows A-D of a 96-well plate at. The Molecular Devices SpectraMax 384 Plus is a UV/Vis spectrophotometer and microplate reader combination for more reliable and fast data reporting with built-in cuvette port and microplate drawer. Molecular Probes 96-well microplates for fluorescence-based assays are black-walled, clear-bottom 96-well microplates that minimize well-to-well crosstalk and. sdale innersh Triangle Woingham ershire RG41 T United Kingdom 11 umolde. Cayman Chemical. (Molecular Devices cat. , Corning, NY) or Greiner. These robust readers have been placed in labs from. Endpoint, kinetic, spectrum. UV cuvettes are ideally suited for measurements at 260 nm, 280 nm and in the visible range. Highly selective for dsDNA over ssDNA or RNA. “The ABS Plus reader comes standard with our patented PathCheck Technology which enables consistent results across a 96- or 384-well plate despite volume differences that may. Fluorescence Microplate Reader Comparison Chart. Products Clone Screening Accessories and Consumables Deep-well Plates Deep-well Plates Deep well blocks available in 24, 96 and 384-well formats Product Options. Spectramax ® Plus microtiter plate reader (Molecular Devices, Sunnyvale, CA) or similar UV-Vis plate reader; SoftMax ® Pro software (Molecular Devices) (optional) UV compatible quartz plate (for example: # R8024; Molecular Devices) or 96-well Costar ® with UV transparent bottom (for example: #3635 ; Corning Inc. The EMax Plus Microplate Reader measures 96-well plates and comes with eight standard filter modes to cover the entire visible range, for applications such as protein quantification, cell viability, cell proliferation and ELISA. SoftMax Pro GxP Software version 5. Service Contracts, Preventative Maintenance and. x. The SpectraMax® iD3 and iD5 Multi-Mode Microplate Readers measure absorbance, fluorescence, and luminescence. Compatible with Molecular Devices SPECTRAmax 190 and 250 plate readers. The instrument reads any standard cuvette, microcuvette, test tube or 96- and 384-well standard or UV transparent microplate over a full 190 to 1000. Resipher 96x Starter Kit for use with 96-well plates Includes: (1) Resipher Data Hub, (1) Resipher 96x Reader, (3) sterile sensing lids, all cables Turns a 96-well plate into a precise dynamic oxygen consumption reader, measures 96 wells Utilizes Lucid Lab Cloud software for connection to any…. FilterMax F3 and F5 Multi-Mode Microplate Readers are filter-based microplate readers offering fluorescence polarization, time-resolved fluorescence, glow luminescence, and other read modes for programmable endpoint, kinetic, multiple-wavelength linear scan and area scan measurements. Supported Plates Microplates having 6, 12, 24, 48, 96, and 384 wells can be used in the. Standard 6-, 12-, 24-, 48-, 96- and 384-well microplates. The luminescence was measured in a SpectraMax. Contents of wells were pipetted up and down several times to mix. Determine concentration using the Beer-Lambert LawPage 1 ™ VERSAmax Tunable Microplate Reader Operator’s Manual for Instruments with Serial Numbers Beginning with BN Molecular Devices Corporation 1311 Orleans Drive Sunnyvale, California 94089 Part # 0112-0084 Rev. Self-righting base, up to 40 degrees past center without dispenser. Detection cartridge modularity allows for easy addition of new read modes within minutes without. An industry-renowned instrument for monitoring GPCRs and ion channels. 001 ABS (displayed), 0. President of Molecular Devices. You should ensure that there is enough volume in compound plate wells to operate the IonWorks® system taking into account the dead volume. x. #R8340) SpectraMax i3x Multi-Mode Microplate Reader (Molecular Devices cat. $3,450. Wells to read. 1 - 32 of 54 Sort View Show > Well Plate Reader Accessory PerkinElmer For measuring the fluorescence or phosphorescence from a 6-, 12-,24-, 48-, or 96-well plate in either the X or Y direction or for luminescence micro. 1. The Molecular Devices SpectraMax® M5/M5e Microplate Reader was tested for compatibility with Life. Each well of a 96- well microtiter plate is coated with a fluorescently labeled IgG-binding peptide, Protein G. TNF standard curve. a white 96-well half-area microplate (Corning cat. measurements in compatible Molecular Devices® microplate readers. White plates reflect the light, enabling even low levels of Luminescence to be detected. Note: SoftMax Pro Software version 6 and 7 also include a searchable help file within the software. White 96-well platePlate formats. 5032338. 303 unable to cal dark current. Click “Next”, and the reader will start scanning the four corner wells; a progress indicator will appear: fter the reader finishes scanning the four corner wells, you will see a screen with an image of the upper left corner A well and a pink crosshair. Linear, Orbital Slow, and Orbital Fast. of 3 wells of a 96-well plate, from 1 of 3 independent experiments. 6, 12, 24, 48, 96, 384-well plates : ANSI/SLAS Light source. The. 96- and 384-well. The trademarks used herein are the property of Molecular Devices, LLC or their respective owners. The Varioskan LUX multimode reader has received HTRF™ certification from Cisbio Bioassays and DLReady™ validation from Promega Corporation. 9. 2 OD at 200 nm; this decreased to 0. All trademarks used herein are the property of. 9000-0756. Look for a washer which can handle a variety of plate types, including deep well plates and 96-, 384- and 1536-well plates. 4 (Molecular Devices cat. Description. The SpectraMax® iD5 Multi-Mode Microplate Reader is the complete laboratory solution to help you increase your research capabilities and comes with built-in absorbance, fluorescence, luminescence, time-resolved fluorescence (TRF), and tunable fluorescence polarization (FP) read modesRobust, high-value microplate readers that can run. 0 fmol/well FITC 200 µL in 96 wells Bottom-read detection limit. Gemini XPS & EM readers measure fluorescence on a variety of sample formats from 6- to 384-well microplates. Our multi-mode plate readers provide great flexibility and include absorbance, fluorescence and luminescence with configurable options for fluorescence polarization (FP), time-resolved fluorescence (TRF), FRET, and AlphaScreen. Using the patented PathCheck® Pathlength Measurement Technology, the SpectraMax M5e Microplate Reader transforms each well in a microplate to a fixed. The Molecular Devices ThermoMax is an efficient, temperature-regulated, 96 well microplate reader. Two days before the experiments, cells are seeded into the clear-bottom 96-well plates (approximately 10,000 cells per well for cancer cell lines and 30,000–40,000 cells per well for fibroblasts and NPC; see Note 5) and cultured in the media suited for users’ cell lines. % transmittance of plates used in a plate reader. Access this by going to. Users can also perform measurements such as endpoint, multiple wavelength, kinetic, linear and area scans. Plate readers, also known as microplate readers or microplate photometers, are instruments which are used to detect biological, chemical or physical events of samples in microtiter plates. The ImageXpress® Pico Automated Cell Imaging System is more than a digital microscope, combining high-resolution imaging with powerful analysis. Reader Documentation Version No. Here, we demonstrate validation of the SpectraMax ® i3x Multi-Mode Microplate Reader for NanoBRET assays. The top- and bottom-reading Gemini EM Microplate. 96 wells 384 wells 1 pM 1 pM Optimized bottom sensitivity (fluorescein) 96 wells 384 wells 2 pM 2.